Fig. 6.
Cytotoxicity assay with purified perforin.
PIG-A mutant and control cells showed similar sensitivity to purified perforin in vitro. Both K562 cells and JY-5 cells with (GPI−) and without (GPI+) PIG-Amutation were incubated for one hour with 6 U purified perforin. Each value represents the mean (± SD) of triplicate assays.