TCR-γ PCR of isolated single CD30⁺ cells and single CD30⁻ cells (polyacrylamide gel stained with ethidium bromide, PAGE 6%).

(A) TCR-γ PCR products obtained with primers covering Vγ gene segments 1 to 8. CD30⁺ cells (lanes 1-5). Initial biopsy of patient no. 2 (lanes 1, 3, 4); biopsy taken 52 months later (lanes 2, 5). Note the biallelic rearrangement in this patient. Simultaneous detection of both rearrangements is only in lane 3, whereas lanes 1 and 2 and lanes 4 and 5 represent either 1 of the 2 rearranged alleles, respectively. Negative control (lane 6). Reactive CD30⁻cells of patient no. 2 (lanes 7, 8). (B) TCR-γ PCR products obtained with primers covering Vγ gene segment 10. CD30⁺ cells of patient no. 8 (lanes 1-5). Negative control (lane 6). Reactive CD30⁻ cells of patient no. 8 (lanes 7, 8).