Fig. 2.
Effects of STI571 on RAB5EP-PDGFBR– andbcr-abl–transformed Ba/F3 cells.
RAB5EP- PDGFBR–transformed (A) andbcr-abl–transformed (B) BaF3 cells were incubated at 2 × 105 cells/mL, with or without murine IL-3 (1 ng/mL), in the presence or absence of STI571 (10 μM). Viable cells were counted daily to assess cell growth, with each treatment being done in triplicate. (C) Dose response for STI571 after 48 hours of incubation for bcr-abl and RAB5EP-PDGFBR. (D) Western blot for phosphorylated (activated) form of STAT1 at baseline and 4 and 8 hours after addition of STI571 (10 μM).
