Tolerance to donor skin grafts and deletion of donor lymphocytes bearing host Mtv-specific VβTCR in complete donor chimeric mice at 24 weeks after DLI.

(A) Donor, recipient, and third-party skin graft survival after DLI. Chimeric animals were specifically tolerant to donor skin grafts. (B) Expression of postnatal donor VβTCR was analyzed at 24 weeks after DLI by 3-color flow cytometry for CD3, relevant VβTCR (Vβ5.1/2, Vβ11, and Vβ12) and donor CD45 isoform (CD45.1, CD45.2). ***P < .001 prenatal donor versus B6 control or postnatal versus B6-LY5.2Cr.