NSD3 and NUP98 exon structures and chimeric transcripts.

(A, B) NSD3 and NUP98 exon structures showing encoded domains. Coding regions are depicted as dark gray; noncoding regions are light gray. Exon lengths are to scale, as indicated by ruler. Primers used in this study are positioned below the respective gene (only the end parts of the names are indicated). (A)NSD3 exon structure. All transcript sequences start at various points in exon 0. Ending points are indicated for the following GenBank accession numbers: (a) (WHSC1L1s), AF332468; (b) (WHSC1L1l),AF332469; (c) (FLJ12498), AK022560; (d) (NSD3S), AJ295992; (e) (NSD3L),AJ295990; (f) (NSD3L2, lacking exon 14), AJ295991. (B) NUP98exon structure, derived from GenBank accession number U41815. Exons 11 to 15 are also indicated as A-E by Arai et al.7 (C-F) Schematic representation of NSD3-NUP98 fusion transcripts.NUP98-derived exons are depicted as light gray;NSD3-derived exons are dark gray. (C) Short-form transcript, containing NUP98 exons 1 through 11 and NSD3exons 4 through 9a. (D) Long-form transcript, containingNUP98 exons 1 through 11 and NSD3 exons 4 through 23. (E) Alternatively spliced long-form transcript, lackingNSD3 exon 14. (F) Reciprocal transcript, containingNSD3 exons 0 through 3 and NUP98 exons 12 through 20.