Fig. 3.
![Fig. 3. HPK1 enhances JNK and, to a lesser extent, ERK and p38 MAP kinase activation in H2O2-treated EL-4 cells. / EL-4 cell lines expressing wt HPK1 (pHA-HPK1 wt), a kinase-inactive K46M mutant (pHA-HPK1 M[46]), AS-HPK1 (pHA-HPK1 AS), or control vector RNA (pEGZ-HA) were treated with 1 mmol/L H2O2 for 0 to 120 minutes. 100 μg cellular proteins were resolved by SDS-PAGE, blotted, and detected with the indicated Abs. Representative blots from 4 independent experiments are shown.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/100/3/10.1182_blood-2002-01-0089/5/h81522889003.jpeg?Expires=1735596110&Signature=Y0bCDS3F-PhdgTfGFZ9D~K5EhX82dKAdpC9C4Bolo2DeTOyNmg8xE4~s339X2Yd66ayP9KACmUNwayHBenZ4BsidCTuUUYIEvD6Ddl1sLYEiKTboUsHELuQaj89sehq7Q6oPjsX9A2mGJLEdpkgUpIeWY78zMA67WTSwlfMBLcZZ~aDwz8AdSGYZcD25CT1VYS8OfMXwclda0WOuje7lGv5DpHcEbtwhWx1fFAXTpdI6IvYBQt7tukMLyutuuFtaV2~ZdBH8ucB7zMNpF20ixh5611IQV48bGMe4M19Oom28e9pXE4s7SimmkUaVIKEfybMPadzgX-eag5dBGOJR-w__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
HPK1 enhances JNK and, to a lesser extent, ERK and p38 MAP kinase activation in H2O2-treated EL-4 cells.
EL-4 cell lines expressing wt HPK1 (pHA-HPK1 wt), a kinase-inactive K46M mutant (pHA-HPK1 M[46]), AS-HPK1 (pHA-HPK1 AS), or control vector RNA (pEGZ-HA) were treated with 1 mmol/L H2O2 for 0 to 120 minutes. 100 μg cellular proteins were resolved by SDS-PAGE, blotted, and detected with the indicated Abs. Representative blots from 4 independent experiments are shown.
