Fig. 5.
Effect of C2-ceramide on iron-inducedc-myc expression by HSFCs.
HSFCs were precultured with 1 mM ferric citrate for 7 days, then transferred to 96-well plates and incubated with 25 μM C2-ceramide in the presence of 1 mM ferric citrate for another 24 hours. Then the cells were harvested and RNA was extracted and examined by RT-PCR. The remainder of the experiment was performed as described in Figure 4. Lane 1, DNA markers; lane 2, HSFCs precultured with ferric citrate then cultured with ferric citrate plus C2-ceramide; lane 3, HSFCs cultured with ferric citrate.
