Fig. 7.
Effects of iron and C2-ceramide on HSFC apoptosis.
HSFCs were cultured as described for Figure 5 and stained as described in “Materials and methods.” HSFCs were examined microscopically for the presence of DNA fragmentation using the ApoAlert assay after iron exposure (A) or iron followed by C2-ceramide for 24 hours (B). Original magnification, × 100.