Comparison of costimulatory and adhesion molecule expression on SJL/J astrocytes and CVEs.

SJL/J astrocytes or CVEs were cultured with no cytokine, 100 U/mL IFN-γ, 500 U/mL TNF-α, or both cytokines for 48 hours. Cells were analyzed for relative expression of B7-1, B7-2, CD40, ICAM-1, and VCAM-1 using flow cytometric analysis. Data are plotted as mean fluorescence shifts (mean fluorescence intensity of the marker − mean fluorescence intensity of the isotype control) for each marker on cells following cytokine pretreatment.