Lymphomyeloid repopulation in NOD-SCID mice transplanted with HOXB4-GFP– and GFP-transduced CB cells.
Three cohorts of NOD/SCID mice were injected with different dilutions of CB cells infected either with the HOXB4 or the GFP virus. (A) FACS analysis of a representative lymphomyeloid-engrafted mouse of the HOXB4 and the GFP cohort after injection of 2.5 × 105CD34+ cells 6 weeks after transplantation. The number of human transduced lymphoid (GFP+/CD19+) and myeloid (GFP+/CD15+) cells is shown. Human lymphomyeloid engraftment was defined as at least 5 GFP+/CD19+ and GFP+/CD15+ cells/2 × 104events.19 (B) CRU frequency among HOXB4-GFP– and GFP-transduced CB cells in NOD/SCID mice. Analysis of BM aspirates and total BM was performed 3 and 6 weeks after transplantation and analyzed with Poisson statistics. *P = .02.
