Western blot of the expressed del791-801 TM.

Transient transfections of COS-7 cells were performed using the wild-type or mutated TM expression vector with a V5 tag sequence attached adjacent to the STOP codons. Proteins in whole cell lysates prepared by addition of Laemmli buffer to cells (A) or in detergent extracts of the cells (B) were separated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) under nonreducing conditions. Immunodetection was performed using an anti-V5 tag antibody.