Flow cytometric analysis of the enrichment of K562 cells expressing recombinant RhD antigen by magnetic-bead sorting.

The human MAb anti-D F5 was used to compare RhD antigen expression on G418-resistant K562/RhD cells before and after magnetic bead sorting, as described in “Materials and methods.” K562/Lu stably expressing the recombinant Lu blood group glycoprotein was used as negative control.