Effect of TPA treatment on the cell-surface expression of RhD, RhAG, and Lu antigens in K562/RhD, K562/RhAG, and K562/Lu transfectants.

Cells were analyzed by flow cytometry before and after TPA treatment for 48 hours. Antibodies included (A) anti-RhD, LOR-15C9 hMAb; (B) anti-RhAG, LA18.18 mMAb; and (C) anti-Lu, LM342 mMAb. Similar results were obtained when RhD expression was analyzed with F5 and BRIC69 MAbs (not shown).