Fig. 8.
![Fig. 8. Immunoprecipitation of RhD and RhAG from K562 and K562/RhD/RhAG cells. / TPA-treated (+) and untreated (−) cells were incubated for 3 hours with [35S]-methionine. Cell lysates from K562 (W.T.) and K562/RhD/RhAG cells were used for immunoprecipitation with the MPC8 anti-Rh polyclonal antibody (PAb) (A) or the LA18.18 anti-RhAG mMAb (B) and Protein A–Sepharose. The isolated proteins were separated by 10% SDS-PAGE under reducing conditions. Low molecular weight proteins from Biorad were used as standards. Arrows indicate the 32-kd Rh and RhAG proteins.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/100/3/10.1182_blood.v100.3.1038/4/h81522885008.jpeg?Expires=1735827270&Signature=FINiSua-YXHi9oPdXQxm6AZiKjbIXT8SjtPScEgdTupcwWfOIWFGMoiwytHfOzYNOiIwqdqBHzERExr5cP9gDHEFI86TE1DnO0maAAEokL3F-j81hvUeMlpNGze6cVVxlmwUEkPUriQGWGrKhGjv9agRklqNjviuPqKcepWW1JzOb9YGe7Vp0bYhfw~-foz~fMykSGTey1yw59cPquSMYdGyBMp0rnoZutYEpHlCVMs5tVtzeiuiFE-1swuVNa4ULK3raFDsOEG813mKM0fp3oAcJal96yQHa6cZ4PvdhOrPK3KFE6JSWqfnnYpXyRB7nhRQO1OsqYgBQffWuBOKoQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Immunoprecipitation of RhD and RhAG from K562 and K562/RhD/RhAG cells.
TPA-treated (+) and untreated (−) cells were incubated for 3 hours with [35S]-methionine. Cell lysates from K562 (W.T.) and K562/RhD/RhAG cells were used for immunoprecipitation with the MPC8 anti-Rh polyclonal antibody (PAb) (A) or the LA18.18 anti-RhAG mMAb (B) and Protein A–Sepharose. The isolated proteins were separated by 10% SDS-PAGE under reducing conditions. Low molecular weight proteins from Biorad were used as standards. Arrows indicate the 32-kd Rh and RhAG proteins.
