Fig. 9.
Fig. 9. Immunolocalization of Rh and RhAG in K562 transfectants. / TPA-treated and untreated cells were incubated with mMAb LA18.18 and hMAb LOR-15C9. MAbs bound to RhAG and RhD polypeptides were probed with Alexa-Fluor 488–conjugated anti-mouse IgG (green fluorescence) and Alexa-Fluor 568–conjugated anti-human IgG (red fluorescence), respectively. Fluorescence images were visualized using a confocal laser microscope. Coexpression of Rh and RhAG resulted in yellow fluorescence on merged images. Because of the small size and high nucleus/cytoplasmic ratio of K562 cells, discriminative fluorescence staining patterns can be interpreted with confidence only for expression or nonexpression of the relevant antigens at the cell membrane.

Immunolocalization of Rh and RhAG in K562 transfectants.

TPA-treated and untreated cells were incubated with mMAb LA18.18 and hMAb LOR-15C9. MAbs bound to RhAG and RhD polypeptides were probed with Alexa-Fluor 488–conjugated anti-mouse IgG (green fluorescence) and Alexa-Fluor 568–conjugated anti-human IgG (red fluorescence), respectively. Fluorescence images were visualized using a confocal laser microscope. Coexpression of Rh and RhAG resulted in yellow fluorescence on merged images. Because of the small size and high nucleus/cytoplasmic ratio of K562 cells, discriminative fluorescence staining patterns can be interpreted with confidence only for expression or nonexpression of the relevant antigens at the cell membrane.

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