Schematic representation of lentiviral vector.

The full-length lentiviral genomic transcript is encoded by a Rous sarcoma virus (RSV)/HIVLTR RU5 chimeric promoter. The vector contains a mutation in the 3′ long terminal repeat (LTR) that is copied onto the 5′ LTR, which results in the self-inactivation of the vector upon transduction. Consequently, only the internal CMVpromoter remains active and expresses the GFP reporter gene. The WPRE, cPPT, CTS,Rev-responsive element (RRE), splice donor and acceptor sites, and residual gag sequence (Δgag) are indicated.