Quantitative analysis of the repopulating capacity of CD34⁺Lin⁻ and CD34⁺CD38⁺Lin⁻ cell populations isolated from G-CSF plus SCF–mobilized PB.

Summary of human cell engraftment in NOD/SCID EMV^null mice that received transplants of (A) G-CSF plus SCF–mobilized CD34⁺Lin⁻ or CD34⁺CD38⁺Lin⁻ cells. Cells were injected into the tail vein of sublethally irradiated NOD/SCID EMV^null mice at the dose ranges indicated. Mouse BM cells were extracted 6 to 8 weeks after transplantation to detect the presence of human cells by flow cytometry. Each symbol (▪) represents a single mouse with CD34⁺Lin⁻ or CD34⁺CD38⁺Lin⁺ transplanted cells derived from a total of 5 independent G-CSF plus SCF–mobilized samples (n = 14). Human engraftment was observed with 100 000 G-CSF plus SCF–mobilized CD34⁺Lin⁻ and CD34⁺CD38⁺Lin⁻ cells. Representative Southern blot analysis of human cell engraftment after transplantation with (B) G-CSF plus SCF–mobilized CD34⁺Lin⁻ and CD34⁺CD38⁻Lin⁻ cells. The level of human engraftment was determined by comparing the characteristic 2.7-kb band to human-to-mouse DNA mixture controls.