Fig. 2. Immunoblot analysis of intact cells directly solubilized in boiling SDS sample buffer. / Blots were probed with antiankyrin and antiprotein 4.1 antibodies. Lane 1, uninfected and ring-infected RBCs. Lane 2, trophozoite- and schizont-infected RBCs. Truncated ankyrin and protein 4.1 of approximately 155 kd and approximately 56 kd, respectively, were detected in trophozoite- and schizont-infected RBCs but not in uninfected or ring-infected RBCs. Note that in the left panel, overexposure of lane 1 did not detect the 155-kd band in uninfected and ring-infected RBCs (lane 1′).
Fig. 2.

Immunoblot analysis of intact cells directly solubilized in boiling SDS sample buffer.

Blots were probed with antiankyrin and antiprotein 4.1 antibodies. Lane 1, uninfected and ring-infected RBCs. Lane 2, trophozoite- and schizont-infected RBCs. Truncated ankyrin and protein 4.1 of approximately 155 kd and approximately 56 kd, respectively, were detected in trophozoite- and schizont-infected RBCs but not in uninfected or ring-infected RBCs. Note that in the left panel, overexposure of lane 1 did not detect the 155-kd band in uninfected and ring-infected RBCs (lane 1′).

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