CD4⁺ and CD8⁺ T-cell proliferation is equally inhibited by BMSCs.

Highly purified CD4⁺ and CD8⁺ T lymphocytes were used as responders in cultures with allogeneic PBL, DCs, or PHA with (black bars) or without (white bars) BMSCs. Addition of BMSCs resulted in a significant inhibition of both CD4⁺ and CD8⁺ cells without statistical differences in all points evaluated (*P = .0005, Student t test for paired data). The percentage of inhibition obtained by using the whole CD2⁺ cell population was significantly higher (**P = .0001, Student t test for paired data). The data are expressed as mean ± SD of triplicates of 3 separate experiments.