Analysis of cytokine-independent cell growth of Ba/F3-derived transfectants.

Parental Ba/F3 cells and other transfectants were washed extensively and resuspended in the media without cytokine at a density of 5 × 10⁴/100 μL complete RPMI and plated in 96-well plates. The cells were cultured for another 48 hours and pulsed with 1 μCi (0.037 MBq) of ³H-TdR for 8 hours, and then³H incorporation was quantified. The number underneath each column represents independent Ba/F3 clone coexpressing v-Abl Δ858-1080 and either v-Akt (A), p110CAAX (B), or v-H-Ras (C), respectively.