Akt, but not p110CAAX or v-H-Ras, complements the efficiency and latency of the v-Abl Δ858-1080 mutant in tumorigenesis.

Nude mice were injected with either parental IL-3–dependent Ba/F3 cells; Ba/F3 transfectants expressing wild type; v-Abl Δ858-1080 mutant; single transfectants that express either v-Akt, p110CAAX, or v-H-Ras; or double transfectants that express v-Abl Δ858-1080 mutant and either v-Akt, p110CAAX, or v-H-Ras. The mice were monitored for visible signs of growth over the 30-day period of after injection. Data from 3 experiments are pooled. The total numbers of mice injected for each category of transfectants were as follows: 2 for parental Ba/F3 cells, 4 for p160 v-Abl–Ba/F3, 4 for Jak1-binding mutant Ba/F3, 6 for Akt-Ba/F3, 4 for Ras-Ba/F3, 4 for p110CAAX-Ba/F3, 10 for Akt/J1BM-Abl-Ba/F3, 10 for Ras/J1BM-Abl-Ba/F3, and 10 for p110CAAX/J1BM-Abl-Ba/F3.