Transduction of human and macaque PBLs.

PBLs of human (A) or cynomolgus macaque (B) origins were transduced with the indicated SIV vector pseudotypes at different MOIs. Human PBLs were activated with soluble anti-CD3 and anti-CD28 antibodies for 24 hours. Macaque PBLs were activated with concanavalin A and recombinant human IL-2 for 2 days prior to infection. Activated PBLs were infected for 4 hours with SIV vectors pseudotyped with VSV-G (triangles), MLV-A GP (closed circles), GALV/TR GP (open circles), or RD114/TR GP (closed squares). Infected cells were washed in PBS and grown in PBL culture medium, and transduction efficiency was assessed 5 days after infection. The results of experiments performed with PBLs from different donors are shown, as well as the statistical analyses of the maximal transduction efficiencies of at least 4 experiments performed with PBLs derived from different donors and stocks of pseudotyped vectors.