Fig. 3.
Fig. 3. Effect of LDL isolated from heme- or metHb-containing plasma on endothelial HO-1 and ferritin. / LDL isolated from heme- or metHb-containing plasma induces endothelial HO-1 and ferritin. (A) For HO-1 mRNA Northern analysis, endothelial cells were incubated with native 50 μg/mL LDL (first lane) or with LDL derived from plasma containing 80 μM heme (second lane), 20 μM ferroHb (third lane), 20 μM metHb (fourth lane), or 80 μM metmyoglobin (fifth lane) as described in “Materials and methods.” (B) Densitometry tracings of HO-1 mRNA bands expressed as arbitrary OD units. (C) The corresponding 28S and 18S ribosomal RNA of the Northern blot in panel A. (D) HO enzyme activity measured at 8 hours after exposure of endothelial cell monolayers to the same LDLs as above. (E) Ferritin protein was measured at 16 hours in endothelium treated as in panel A. Results represent mean ± SE of at least 3 experiments performed in duplicate.

Effect of LDL isolated from heme- or metHb-containing plasma on endothelial HO-1 and ferritin.

LDL isolated from heme- or metHb-containing plasma induces endothelial HO-1 and ferritin. (A) For HO-1 mRNA Northern analysis, endothelial cells were incubated with native 50 μg/mL LDL (first lane) or with LDL derived from plasma containing 80 μM heme (second lane), 20 μM ferroHb (third lane), 20 μM metHb (fourth lane), or 80 μM metmyoglobin (fifth lane) as described in “Materials and methods.” (B) Densitometry tracings of HO-1 mRNA bands expressed as arbitrary OD units. (C) The corresponding 28S and 18S ribosomal RNA of the Northern blot in panel A. (D) HO enzyme activity measured at 8 hours after exposure of endothelial cell monolayers to the same LDLs as above. (E) Ferritin protein was measured at 16 hours in endothelium treated as in panel A. Results represent mean ± SE of at least 3 experiments performed in duplicate.

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal