Fig. 1.
![Fig. 1. Staining intensity of different leukocyte populations with OX1 and anti-RT7a mAb. / LEW.1W peripheral leukocytes (obtained from whole EDTA blood samples, erythrocytes lysed by NH4Cl) were double-stained with specific antibodies (R73/T cells, OX33 B cells, 3.2.3/NK cells, and HIS48/granulocytes; indirect staining with phycoerythrin-conjugated goat anti-mouse mAb (GAM) and OX1 or anti-RT7a mAb (RT7.1 fluorescein isothiocyanate–[FITC] or OX1bio plus FITC-conjugated streptavidin, direct staining), respectively. FL1/FL2 dot plots were gated for subpopulations of total leukocytes. Representative histograms for gated cells are shown for OX1 and anti-RT7a mAb staining intensities, and mean channels of fluorescence are indicated.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/99/10/10.1182_blood.v99.10.3566/6/h81022544001.jpeg?Expires=1735828189&Signature=PzkoEGK7VSeCElJSOgpCkR4pYuwaOIZEGw5XUu5l0hASJZ-DDisy2Fr8UNf8NaeAEXAaXFMvM2WU5iuYKEFblgBJk7OaksyIphIXKSPOZqqtzuDhcbbem-ER1ILXx0SQ-hFJ4HeP-rlMTpRE4J9tAO4K7aIhLwgLc8rZKY~iYC~7fW-etlVvA6vFr70-af33mJJ5JJYPsvUDTVgxPkDv4prCIC41SG4jswvDT0iGqBTSVll7NjpeKf3dqq~-t3ZzycGrNowXHoIb~WmFyFg45DpoHHe2NELMCoIjAg8rp4Z0EQ4wUPlasV5MjW3jsSygoOhn0oEONKNocSACXVoLJw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Staining intensity of different leukocyte populations with OX1 and anti-RT7a mAb.
LEW.1W peripheral leukocytes (obtained from whole EDTA blood samples, erythrocytes lysed by NH4Cl) were double-stained with specific antibodies (R73/T cells, OX33 B cells, 3.2.3/NK cells, and HIS48/granulocytes; indirect staining with phycoerythrin-conjugated goat anti-mouse mAb (GAM) and OX1 or anti-RT7a mAb (RT7.1 fluorescein isothiocyanate–[FITC] or OX1bio plus FITC-conjugated streptavidin, direct staining), respectively. FL1/FL2 dot plots were gated for subpopulations of total leukocytes. Representative histograms for gated cells are shown for OX1 and anti-RT7a mAb staining intensities, and mean channels of fluorescence are indicated.
