American Society of Hematology

Fig. 7.

$Fig. 7. Chimerism of LEW.1W-7B rats after 7 Gy γ-irradiation and BM transplantation from LEW.1W rats treated with a high dose of anti-RT7a mAb. / LEW.1W rats (n = 4) were treated with a high anti-RT7amAb dose (see text; Figure 4) on day −1. On day 0, LEW.1W-7B recipients were irradiated with 7 Gy γ-irradiation. Six hours later, one recipient received all BM cells that could be obtained from one donor animal. Leukocytes of whole blood samples from LEW.1W-7B recipients (gained as described) were stained with the respective mAbs (Figure 1). Patterns of chimerism are shown for T cells (panel A), B cells (panel B), and granulocytes (panel C). RT7a-positive donor cells from pretreated animals are shown as fractions of total T cells, B cells, and granulocytes as solid black boxes, whereas RT7b-positive recipient cells of irradiated animals are shown as gray boxes.$

Chimerism of LEW.1W-7B rats after 7 Gy γ-irradiation and BM transplantation from LEW.1W rats treated with a high dose of anti-RT7^a mAb.

LEW.1W rats (n = 4) were treated with a high anti-RT7^amAb dose (see text; Figure 4) on day −1. On day 0, LEW.1W-7B recipients were irradiated with 7 Gy γ-irradiation. Six hours later, one recipient received all BM cells that could be obtained from one donor animal. Leukocytes of whole blood samples from LEW.1W-7B recipients (gained as described) were stained with the respective mAbs (Figure 1). Patterns of chimerism are shown for T cells (panel A), B cells (panel B), and granulocytes (panel C). RT7^a-positive donor cells from pretreated animals are shown as fractions of total T cells, B cells, and granulocytes as solid black boxes, whereas RT7^b-positive recipient cells of irradiated animals are shown as gray boxes.

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