Fig. 1.
Fig. 1. cDNA restriction map and RT-PCR-RFLP analysis for theAbl Thr315Ile mutation. / The Thr315Ile mutation was investigated by studying the loss of Dde I restriction enzyme site. (A) Dde I restriction map of the F2R2-412 bp cDNA PCR analyzed fragments. Restriction sites are indicated by arrows. The cryptic Dde I site is shown by (*). Length of corresponding fragments obtained after complete digestion are indicated below. (B) Corresponding RFLP pattern on 2.5% ethidium-bromide–stained agarose gel. (-) indicates cDNA PCR fragment prior to Dde I digestion; (+), cDNA PCR fragment after complete Dde I digestion. A1, B1, C1: RFLP pattern obtained from patients A, B, and C, respectively, at diagnosis. A2, B2, C2: RFLP pattern obtained from patients A, B, and C, respectively, at time of resistance. Blk indicates water RT-PCR negative control.

cDNA restriction map and RT-PCR-RFLP analysis for theAbl Thr315Ile mutation.

The Thr315Ile mutation was investigated by studying the loss of Dde I restriction enzyme site. (A) Dde I restriction map of the F2R2-412 bp cDNA PCR analyzed fragments. Restriction sites are indicated by arrows. The cryptic Dde I site is shown by (*). Length of corresponding fragments obtained after complete digestion are indicated below. (B) Corresponding RFLP pattern on 2.5% ethidium-bromide–stained agarose gel. (-) indicates cDNA PCR fragment prior to Dde I digestion; (+), cDNA PCR fragment after complete Dde I digestion. A1, B1, C1: RFLP pattern obtained from patients A, B, and C, respectively, at diagnosis. A2, B2, C2: RFLP pattern obtained from patients A, B, and C, respectively, at time of resistance. Blk indicates water RT-PCR negative control.

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