Fig. 4.
Nucleotides are chemotactic for immature but not mature DCs.
Immature and mature DCs were exposed for 90 minutes at 37°C in a Transwell chamber to the indicated concentrations of ATP (A), UTP (B), ADP (C), 2-MeSATP (D), αβ-meATP (E), or BzATP (F). C5a (10−8 M) as a positive control for immature DCs and MIP-3β (10−8 M) as a positive control for mature DCs provoke at optimal concentration an increase of the chemotactic index of 1.85 ± 0.18 and 2.04 ± 0.27, respectively. The chemotactic index is calculated as the ratio between the number of migrated cells in the presence or in the absence of the nucleotide. Data are expressed as means SEM (n = 4).
