Analysis of HHV-8 lytic cycle RNAs (T1.1, K8.1, and ORF74) on sequential days after primary infection.

(A) At top is the detection of T1.1 by RNA blot hybridization of 10 μg total RNA from fMVDECs harvested before infection, on days 1 through day 10 after primary infection, and on day 19 (9 days after passage). At bottom, the blot was reprobed to detect cellular GAPDH. The position of 18S RNA (2.37 kb) is indicated on the left. (B) At top is the detection of K8.1 RNA before infection and on days 1 through 10 and on day 19 after infection by the same methods described in panel A. At bottom, the blot was reprobed to detect cellular GAPDH RNA. The position 18S RNA is indicated on the left. (C) At top, ORF74 transcripts were detected in 0.5 μg total RNA from uninfected (day 0) and infected (days 1-8) fMVDECs or from BCBL-1 cells (control, right lane) by RT-PCR. Amplification products were detected by DNA blot hybridization. Positions of cDNAs are indicated in base pairs on the left. GAPDH transcripts were detected under identical conditions in the same experiment. An ethidium bromide stain of the gel (GAPDH) is also displayed.