Analysis of HHV-8 latent-cycle ORF72 (vCYC) RNA (encoded by LT1 and LT2) and comparison with lytic T1.1 RNA on days 3 and 8 after primary infection of fMVDECs.

(A) Detection of T1.1 RNA by Northern blot hybridization as described in Figure 4, before infection and on days 1 through 3 and on day 8 after infection. BCBL-1 RNA was analyzed as a control. Positions of 28S (6.33 kb) and 18S RNA (2.37 kb) are indicated on the left. (B) At top, the blot was reprobed with a cDNA encoding full-length ORF72. The positions of LT1 and LT2 transcripts and of residual T1.1 are indicated on the right. Positions of 28S and 18S RNAs are indicated on the left. At bottom, the blot was reprobed to detect cellular GAPDH RNA.