Profiles of ILT2/CD85j expression on CD4⁺ PBL from SS patients in comparison with a representative healthy control patient.

Freshly isolated PBMC from SS patients with cytomorphological data for blood involvement were analyzed by 2-color immunofluorescence and flow cytometry with the ILT2/CD85j-specific mAb GHI/75 (shaded histograms) or an isotype-matched control mAb (unfilled histograms), followed by FITC-conjugated anti-IgG + IgM GAM. After extensive washing to remove the excess of GAM and blocking with normal mouse Ig, a TRI-conjugated anti-CD4 mAb was added. CD4⁺ gated lymphocytes are represented on the histograms. The same staining performed with PBMC from a representative healthy control patient is shown in the histogram and the dot plot to the right.