DCs incorporating CHP/HER2-147 induce HER2p63-specific CD8⁺ T cells.

(A) HLA-A2402⁺ PBMCs were stimulated with autologous DCs either incorporating CHP/HER2-147 or pulsed with HER2p63 for 3 weeks at 1-week intervals. One, 2, and 3 weeks later, 5 × 10⁴CD8⁺ T cells and either of 1 × 10⁵ HER2p63- or HER2p780-pulsed T2-A24 cells were placed in each well of the ELISPOT plate. After 18 hours, IFN-γ spot-forming cells were assessed, as described in “Materials and methods.” Each bar represents the average of duplicates. (B) HLA-A2402⁺ PBMCs were stimulated with autologous immature DCs incorporating CHP/HER2-147 or pulsed with HER2p63 or autologous mature DCs pulsed with HER2p63 for 2 weeks at 1-week intervals. Fifty thousand CD8⁺ T cells and either 1 × 10⁵ HER2p63- or HER2p780-pulsed T2-A24 cells were placed in each well of the ELISPOT plate. After 18 hours, IFN-γ spot-forming cells were assessed, as described in “Materials and methods.” Each bar represents the average of duplicate measurements.