CD4+ clones recognize HER2-derived peptides.
(A) Position and amino acid sequences of 25-mer peptides derived from HER2 protein. (B) Mitomycin C–treated spleen cells (5 × 106) were reacted with 10 μM 25-mer peptides for 1 hour at room temperature and an additional hour at 37°C and were used as stimulator cells. Mitomycin C–treated spleen cells (5 × 106) were also incubated with 20 μg/mL CHP/HER2-147 or CHP/CAB for 3 hours at 37°C, washed, recultured for 18 hours, and used as stimulator cells. Furthermore, 1 × 105 cells/well CD4+ T cell clones A6, A8, and C8 were cultured with 5 × 105stimulator cells/well for 5 days. Proliferation of responding cells was measured using 3H-TdR uptake assay. Each bar represents the average of duplicate measurements.
