Southern blot analysis of subtracted LRH cDNA library.

Pooled plasmids from the presubtracted (pre) and postsubtracted (post) libraries were double-digested with BamHI/EcoR1 and analyzed by Southern blot. The probes were as follows: EST 9063, derived from the driver pool (panel A); superoxide dismutase (SOD), derived from the driver pool (panel B); and Mel-18, derived from subtracted LRH cDNA library (panel C). The bar graphs present quantitation of the data, revealing the effective elimination of the representative driver sequences and enrichment of the selected clones not present in the driver.