Graphical representation of the change in gene expression over time in EML cells treated with ATRA/IL-3.
(A) (B) Each line represents a different gene. The time points (x-axis) represent 0, 6, 24, and 72 hours. The last point on this axis represents EPRO cells, a stage in differentiation beyond 72 hours. The y-axis shows the relative change in gene expression level as log2 (induced/uninduced). Thus, a value of +1 represents a 2-fold increase, and −1 equals a decrease to 50% of the value at time zero. (C) Quantitation of fold change in gene expression (as a ratio of induced/uninduced), for 4 novel genes and (Gapd),derived from microarray data, for 0 hours, 6 hours, and 72 hours of induction, and for EPRO cells. (D) Northern blot to confirm microarray data obtained with RNA from EML cells. Presented are hybridizations with 4 novel clones from our subtracted myeloid cDNA library, as well as Gapd for normalization. Also shown is the ethidium bromide staining of 28S RNA. RNAs are from EML cells treated with ATRA plus IL-3 for 0, 6, 12, and 24 hours to induce myeloid differentiation as well as from EPRO cells.
