Effect of attenuation of β-catenin signaling on proliferation of leukemic cell lines.

(A) Jurkat, (B) HL-60, (C) HUT-102, and (D) K562 cells were transfected with pEGFP (5 μg) and other constructs (10 μg each) as indicated, and GFP-positive cells were isolated by flow cytometry as described in “Materials and methods.” Cells were seeded in 96-well plates at 3000 cells per well and incubated at 37°C. The number of viable cells per well was determined by counting trypan blue–excluding cells in a hemocytometer.