Fig. 4.
Detergent solubility of stomatin and flotillin-2.
Platelets (2 × 109 cells/mL) were incubated at 4°C with lysis buffer containing 1% of Lubrol, CHAPS, TX-100, NP-40, or 60 mM OG. Insoluble material was formed into pellets (by centrifugation at 100 000g for 1 hour), and equal amounts of the resuspended pellet (P) and the supernatant (SN) were analyzed by SDS-PAGE and Western blotting. Representative data are shown (n = 3).
