American Society of Hematology

Fig. 6.

$Fig. 6. Comparison of lipid rafts isolated from TX-100 and CHAPS lysates. / (A,B) Platelets (2 × 109 cells/mL) were lysed in 1% TX-100 or CHAPS at 4°C, and flotation experiments were performed as described in the legends for Figures 3 and 5. Aliquots of the pooled floating (F) fractions 1 to 3 and dense (D) fraction 5 were analyzed. For Western blot analysis (B), equal volumes were loaded on the gel, whereas for silver staining (A), the fraction 5 was diluted 1:30 because of the high protein content. Bars in panel A indicate molecular-weight markers of 250, 150, 100, 75, 50, 37, and 25 kd. Representative data are shown (n = 3).$

Comparison of lipid rafts isolated from TX-100 and CHAPS lysates.

(A,B) Platelets (2 × 10⁹ cells/mL) were lysed in 1% TX-100 or CHAPS at 4°C, and flotation experiments were performed as described in the legends for Figures 3 and 5. Aliquots of the pooled floating (F) fractions 1 to 3 and dense (D) fraction 5 were analyzed. For Western blot analysis (B), equal volumes were loaded on the gel, whereas for silver staining (A), the fraction 5 was diluted 1:30 because of the high protein content. Bars in panel A indicate molecular-weight markers of 250, 150, 100, 75, 50, 37, and 25 kd. Representative data are shown (n = 3).

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