Fig. 7.
Enrichment of stomatin in microvesicles after platelet activation.
Platelets (1 × 109 cells/mL) were activated by incubation with 4 μM ionophore A23187 (A) or 1 U/mL thrombin (B) at 37°C for 30 minutes. Platelets and microvesicles were isolated by differential pelleting steps, and aliquots normalized to equal protein content were analyzed by Western blotting. Representative data are shown (n = 3); pl indicates platelets; and mv, microvesicles.
