Formation and repair of specific DNA adducts in CD34⁺ and CD34⁻ cells after exposure to melphalan or cisplatin ex vivo.

Cells were isolated from CB and exposed to melphalan (5 μg/mL) for one hour or cisplatin (10 μg/mL) for 2 hours and then replaced to fresh medium. Cell aliquots were taken at different time points after treatment and specific DNA adducts were measured by ICA analysis using anti–melphalan-DNA or anti–cisplatin (G-G) antibodies as described in “Materials and methods.”