The effect of DMSO on decreasing frataxin protein levels is not Friend cell clone specific.

Western blot analysis was performed using proteins extracted from the Friend 707 and 745 cell lines incubated in the presence (+) or absence (−) of 1.5% DMSO for 120 hours at 37°C. The membrane was sequentially probed with antifrataxin and anti-β-actin antibodies (see “Materials and methods” for details). Densitometric analysis of the results normalized to the β-actin loading control are shown below the blot. The result illustrated is a typical experiment from 4 experiments performed.