Fig. 4.
MEK1(E) increases eosinophil survival after GM-CSF mRNA transfection.
Eosinophils were transduced for 2 hours with 10 nM MEK1(E) or MKK3b(E) and transfected with GM-CSF mRNA by using the Gene-Gun. One hour after transfection, the cell cultures were diluted by 10-fold (1 × 106 cells/mL), and the survival was determined 4 days later by trypan blue. The survival for transduced pbeos was compared with the nontransduced control and calculated as follows: (% survival with TatMKKinase/% survival with transfected but nontransduced cells) − 1 × 100. Data were analyzed by paired Student t test.
