Phenotype and T-cell stimulatory capacity of DCs developing from EBV- or mock-infected monocytes after 7 days of culture with GM-SCF and IL-4.

(A) Cells were recovered from mock (control) or EBV-infected cultures on day 7, and surface expression of indicated markers was analyzed by immunostaining and FACS analysis. (B) Uninfected (control), mock-infected (adsorbed EBV), or EBV-infected monocytes were cultured for 7 days in the presence of the lymphokines, harvested, irradiated, and used as stimulators at the indicated stimulator-responder ratios in allogeneic mixed-lymphocyte cultures. [³H]-Thymidine incorporation by stimulated T cells was measured after 7 days, as described in “Materials and methods.” In this experiment, the loss of DCs in the EBV-infected cultures was approximately 65% compared with the control cells. Results from 1 of 8 representative experiments are shown.