Recruitment of

CASP10 mutants to DISC. The 293 cells were cotransfected with expression constructs for human Myc-Fas and Flag-FADD either with the Myc-Q257stop mutant (A) or the Myc-A414V mutant (B) as indicated. The transfected cells were harvested and lysed 2 days later and immunoprecipitated with anti-Flag antibodies and sequentially immunoblotted with anti-Myc antibodies as indicated. Aliquots of the same lysates (normalized for total protein content) were also analyzed directly by SDS-PAGE/immunoblotting as indicated. IP indicates immunoprecipitation; WB, Western blotting.