American Society of Hematology

Fig. 3.

$Fig. 3. VCAM-1, but not P- or E-selectin, mediates rolling of FL HPCs in irradiated BM. / Effect of blocking mAbs (100 μg/mouse) on rolling frequency was assessed at 3 hours (A) or 48 hours (B) after irradiation. Rolling fractions after mAb treatment were normalized to rolling fractions determined in the same vessel before mAb application (control). Blocking mAb to VCAM-1 inhibited rolling by 67% at 3 hours and by 56% at 48 hours. (C) Rolling of FL HPCs of WT and FucT−/−mice in normal and irradiated BM. Mutant and WT FL cells were injected sequentially, and rolling was compared in the same venules. Data are presented as the ratio of rolling fractions of FucT−/−versus WT FL HPCs. Thus, a ratio of 1 indicates that both subsets rolled with equal frequency, whereas a ratio of 0.5 indicates that WT FL HPCs rolled twice as frequently as FucT−/− FL HPCs. Bars represent mean ± SEM.$

VCAM-1, but not P- or E-selectin, mediates rolling of FL HPCs in irradiated BM.

Effect of blocking mAbs (100 μg/mouse) on rolling frequency was assessed at 3 hours (A) or 48 hours (B) after irradiation. Rolling fractions after mAb treatment were normalized to rolling fractions determined in the same vessel before mAb application (control). Blocking mAb to VCAM-1 inhibited rolling by 67% at 3 hours and by 56% at 48 hours. (C) Rolling of FL HPCs of WT and FucT^−/−mice in normal and irradiated BM. Mutant and WT FL cells were injected sequentially, and rolling was compared in the same venules. Data are presented as the ratio of rolling fractions of FucT^−/−versus WT FL HPCs. Thus, a ratio of 1 indicates that both subsets rolled with equal frequency, whereas a ratio of 0.5 indicates that WT FL HPCs rolled twice as frequently as FucT^−/− FL HPCs. Bars represent mean ± SEM.

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