Donor-specific skin graft prolongation in porcine hematopoietic chimeras reconstituted with T- and B-cell–depleted B10.D2/o-Tg mouse BMCs.

Survival is shown for skin grafts from the porcine BMC donor (solid line) and third-party B10.RIII mice (dashed line) in NOD/SCID-Tg mice receiving transplants with porcine cells only (A; n = 2), B10.D2/o-Tg mouse BMC-reconstituted NOD/SCID-Tg mice without pretransplantation of porcine cells (B; n = 2), and B10.D2/o-Tg mouse BMC-reconstituted NOD/SCID-Tg mice that had received porcine BMCs prior to murine BMC reconstitution (C; n = 11). Based on the level of porcine chimerism in the WBCs at 1 week prior to B10.D2/o-Tg mouse BMC reconstitution (6 weeks after porcine BMT), mice in panel C are divided into 2 groups: porcine hematopoietic chimeras (▪ with ≥ 1.5% [1.5%-33%] WBC chimerism; n = 8) and mice with poor porcine chimerism (■ with undetectable or < 1.5% WBC chimerism; n = 3). Porcine chimerism was determined by FACS analysis using an antipig pan-tissue mAb. Because the rejection of third-party skin grafts was virtually identical between porcine hematopoietic chimeras and mice with poor porcine chimerism, combined third-party rejection results for the 2 groups are shown (C;--▾--). Results from 2 similar experiments are combined.