Lack of antiporcine donor MLR responses in B10.D2/o-Tg mouse BMC-reconstituted porcine hematopoietic chimeras.

Splenocytes were prepared from NOD/SCID-Tg mice receiving transplants with porcine cells only (I; n = 4), B10.D2/o-Tg mouse BMCs only (II; n = 5), or porcine cells plus B10.D2/o-Tg mouse BMCs (III/IV; n = 11) at week 17 or week 20 after reconstitution with B10.D2/o-Tg mouse BMCs, and antiporcine donor (A) and third-party B10.RIII (B) MLR responses were determined. The recipients of porcine cells plus B10.D2/o-Tg mouse BMCs are divided into porcine hematopoietic chimera (III; n = 8) and poor porcine chimerism (IV; n = 3) groups according to the level of porcine chimerism in the WBCs at 1 week prior to injection of B10.D2/o-Tg mouse BMCs (see the legend to Figure 3). Because the anti–third-party MLR response was virtually identical between porcine hematopoietic chimeras and mice with poor porcine chimerism, combined results for the 2 groups are shown (Figure 4B; III/IV). Data are presented as group means ± SD. *P < .05 for the indicated group compared to group I and to group III in panel A, and to group I in panel B. Results of 2 similar experiments are combined.