Lack of antiporcine donor IgG antibodies in sera of B10.D2/o-Tg mouse BMC-reconstituted porcine hematopoietic chimeras that accepted porcine donor skin grafts.

(A) Levels of antiporcine donor IgG antibodies in sera collected at 10 weeks after reconstitution with B10.D2/o-Tg mouse BMCs (8 weeks after skin grafting). Antipig IgGs were measured by staining of porcine donor PBMCs with 2 or 10 μL serum and antibody levels are presented as MFI. The MFI level correlated directly with the volume of antibody-positive serum used for staining in each instance. Data are shown for the MFI levels of porcine PBMCs bound by 10 μL serum (group means ± SD) in NOD/SCID-Tg mice given transplants with porcine cells only (I; n = 4), B10.D2/o-Tg mouse BMCs only (II; n = 5), or with porcine cells plus B10.D2/o-Tg mouse BMCs that accepted (III; n = 5) or rejected (IV, n = 6) the skin graft of the porcine donor. The background level of MFI (ie, MFI of porcine PBMCs stained with FITC-conjugated secondary mAb only) is indicated as a dotted line. *P < .05 for antibody levels in the indicated group compared to the levels of the same IgG isotype in group I and in group III. Results from 2 similar experiments are combined. (B) Serum levels of total murine IgG in representative samples from group III (○; n = 3), group IV (●; n = 2), and normal B10.D2/o-Tg mice (bar, mean ± SD, n = 3) are shown.