IGF-I induction of phosphorylation of FKHRL1, p70S6 kinase, and GSK3β in other MM cell lines.

IGF-I induces phosphorylation of FKHRL1, p70S6 kinase, and GSK3β via the PI-3K pathway in other MM cell lines. Cells from MM144, OPM-2, and RPMI8226 were cultured in media without serum for 16 hours and left untreated (panel A) or treated with the PI-3K inhibitor LY294002 (20 μM) (panels B, C). Cells were then stimulated with IGF-I (100 ng/mL) for 5 minutes and lysates were analyzed as in Figure 1 with the use of the indicated antibodies.