Fig. 4.
Role of secreted ADP in thrombin-induced Gisignaling.
Effect of α-thrombin (0.5 U/mL) on 10 μM PGE1-stimulated cAMP levels was determined (A) in the presence or absence of Ro 31-8220 (10 μM) and/or AR-C compound in human platelets (as indicated) in normal platelets or in P2Y12-deficient patient platelets (as marked) and (B) in platelets from mice lacking the P2Y12 receptor and wild-type mice. Each data point is the mean ± SE of at least 3 experiments. The experiments with human platelets were repeated at least 3 times using platelets from different donors. *P < .05 by Student t test when compared with the value of PGE1. The experiments with the patient platelets were carried out only once due to problems with the patient's health.
