AYPGKF-induced platelet aggregation in the P2Y12 receptor–deficient mouse platelets.

Platelet aggregation in the washed mouse platelets was determined as described in “Materials and methods.” AYPGKF-induced platelet aggregation was determined in platelets from P2Y12 receptor–deficient mice (dashed line) or wild-type littermates (solid line) using varying concentrations of the agonist. The maximum response (210 seconds after the addition of the agonist) was taken as 100%, and the remaining data were normalized to this value. The data (mean ± SE) are obtained from at least 3 independent experiments.